pVSV Venus VSV-G

LOCUS       pVSV Venus VSV-G              15045 bp ds-DNA     circular SYN 06-AUG-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pVSV Venus VSV-G
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15045)
  AUTHORS   Beier KT, Saunders A, Oldenburg IA, Miyamichi K, Akhtar N, Luo L, 
            Whelan SP, Sabatini B, Cepko CL
  TITLE     Anterograde or retrograde transsynaptic labeling of CNS neurons with
            vesicular stomatitis virus vectors.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Sep 13;108(37):15414-9. Epub 2011 Aug
            8.
  PUBMED    21825165
REFERENCE   2  (bases 1 to 15045)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..15045
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      85..94
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             91..810
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(136..157)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(397..416)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     744..765
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3886..5421
                     /codon_start=1
                     /product="vesicular stomatitis virus G glycoprotein"
                     /label=VSV-G
                     /note="Indiana strain"
                     /translation="MKCLLYLTFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSD
                     LNWHNDLIGTALQVKMPKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPS
                     VEQCKESIEQTKQGTWLNPGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQ
                     FINGKCSNYICPTVHNSTTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFR
                     SNYFAYETGGKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQT
                     SVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLK
                     YFETRYIRVDIAAPILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFP
                     LYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWF
                     SSWKSSIASFFFIIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK"
     primer_bind     complement(12113..12131)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      12127..12174
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(12242..12260)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(12243..12260)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(12550..12569)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(12779..12798)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     12898..12920
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(12958..12976)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        13044..13148
                     /gene="bla"
                     /label=AmpR promoter
     CDS             13149..14009
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(13367..13386)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      14180..14768
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14669..14688
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     14922..14939
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        15027..15045
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 acgaagacaa acaaaccatt attatcatta aaaggctcag gagaaacttt aacagtaatc
       61 agaattctcg aggatccacc ggtcgccacc atggtgagca agggcgagga gctgttcacc
      121 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
      181 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt aatctgcacc
      241 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttaggcta cggcctgcag
      301 tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
      361 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
      421 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
      481 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
      541 gtctatatca cggccgacaa gcagaagaac ggcatcaagg cgaacttcaa gatccgccac
      601 aacatcgagg acggcggcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
      661 gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccgc cctgagcaaa
      721 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
      781 actctcggca tggacgagct gtacaagtaa agcggccgcc ctcctcaggt gctggccata
      841 tgaaaaaaac taacagtaat caaaatgtct gttacagtca agagaatcat tgacaacaca
      901 gtcatagttc caaaacttcc tgcaaatgag gatccagtgg aatacccggc agattacttc
      961 agaaaatcaa aggagattcc tctttacatc aatactacaa aaagtttgtc agatctaaga
     1021 ggatatgtct accaaggcct caaatccgga aatgtatcaa tcatacatgt caacagctac
     1081 ttgtatggag cattaaagga catccggggt aagttggata aagattggtc aagtttcgga
     1141 ataaacatcg ggaaagcagg ggatacaatc ggaatatttg accttgtatc cttgaaagcc
     1201 ctggacggcg tacttccaga tggagtatcg gatgcttcca gaaccagcgc agatgacaaa
     1261 tggttgcctt tgtatctact tggcttatac agagtgggca gaacacaaat gcctgaatac
     1321 agaaaaaagc tcatggatgg gctgacaaat caatgcaaaa tgatcaatga acagtttgaa
     1381 cctcttgtgc cagaaggtcg tgacattttt gatgtgtggg gaaatgacag taattacaca
     1441 aaaattgtcg ctgcagtgga catgttcttc cacatgttca aaaaacatga atgtgcctcg
     1501 ttcagatacg gaactattgt ttccagattc aaagattgtg ctgcattggc aacatttgga
     1561 cacctctgca aaataaccgg aatgtctaca gaagatgtaa cgacctggat cttgaaccga
     1621 gaagttgcag atgaaatggt ccaaatgatg cttccaggcc aagaaattga caaggccgat
     1681 tcatacatgc cttatttgat cgactttgga ttgtcttcta agtctccata ttcttccgtc
     1741 aaaaaccctg ccttccactt ctgggggcaa ttgacagctc ttctgctcag atccaccaga
     1801 gcaaggaatg cccgacagcc tgatgacatt gagtatacat ctcttactac agcaggtttg
     1861 ttgtacgctt atgcagtagg atcctctgcc gacttggcac aacagttttg tgttggagat
     1921 aacaaataca ctccagatga tagtaccgga ggattgacga ctaatgcacc gccacaaggc
     1981 agagatgtgg tcgaatggct cggatggttt gaagatcaaa acagaaaacc gactcctgat
     2041 atgatgcagt atgcgaaaag agcagtcatg tcactgcaag gcctaagaga gaagacaatt
     2101 ggcaagtatg ctaagtcaga atttgacaaa tgaccctata attctcagat cacctattat
     2161 atattatgct acatatgaaa aaaactaaca gatatcatgg ataatctcac aaaagttcgt
     2221 gagtatctca agtcctactc tcgtctagat caggcggtag gagagataga tgagatcgaa
     2281 gcacaacgag ctgaaaagtc caattatgag ttgttccaag aggacggagt ggaagagcat
     2341 actaggccct cttattttca ggcagcagat gattctgaca cagaatctga accagaaatt
     2401 gaagacaatc aaggcttgta tgtaccagat ccggaagctg agcaagttga aggctttata
     2461 caggggcctt tagatgacta tgcagatgag gacgtggatg ttgtattcac ttcggactgg
     2521 aaacagcctg agcttgaatc cgacgagcat ggaaagacct tacggttgac attgccagag
     2581 ggtttaagtg gagagcagaa atcccagtgg cttttgacga ttaaagcagt cgttcaaagt
     2641 gccaaacact ggaatctggc agagtgcaca tttgaagcat cgggagaagg ggtcatcata
     2701 aaaaagcgcc agataactcc ggatgtatat aaggtcactc cagtgatgaa cacacatccg
     2761 taccaatcag aagccgtatc agatgtttgg tctctctcaa agacatccat gactttccaa
     2821 cccaagaaag caagtcttca gcctctcacc atatccttgg atgaattgtt ctcatctaga
     2881 ggagaattca tctctgtcgg aggtaacgga cgaatgtctc ataaagaggc catcctgctc
     2941 ggtctgaggt acaaaaagtt gtacaatcag gcgagagtca aatattctct gtagactatg
     3001 aaaaaaagta acagatatca caatctaagt gttatcccaa tccattcatc atgagttcct
     3061 taaagaagat tctcggtctg aaggggaaag gtaagaaatc taagaaatta gggatcgcac
     3121 caccccctta tgaagaggac actagcatgg agtatgctcc gagcgctcca attgacaaat
     3181 cctattttgg agttgacgag atggacacct atgatccgaa tcaattaaga tatgagaaat
     3241 tcttctttac agtgaaaatg acggttagat ctaatcgtcc gttcagaaca tactcagatg
     3301 tggcagccgc tgtatcccat tgggatcaca tgtacatcgg aatggcaggg aaacgtccct
     3361 tctacaaaat cttggctttt ttgggttctt ctaatctaaa ggccactcca gcggtattgg
     3421 cagatcaagg tcaaccagag tatcacgctc actgcgaagg cagggcttat ttgccacata
     3481 ggatggggaa gacccctccc atgctcaatg taccagagca cttcagaaga ccattcaata
     3541 taggtcttta caagggaacg attgagctca caatgaccat ctacgatgat gagtcactgg
     3601 aagcagctcc tatgatctgg gatcatttca attcttccaa attttctgat ttcagagaga
     3661 aggccttaat gtttggcctg attgtcgaga aaaaggcatc tggagcgtgg gtcctggatt
     3721 ctatcagcca cttcaaatga gctagtctag cttccagctt ctgaacaatc cccggtttac
     3781 tcagtctctc ctaattccag cctttcgaac aactaatatc ctgtcttttc tatccctatg
     3841 aaaaaaacta acagagatcg atctgtttcc ttgacacgcg tcacgatgaa gtgccttttg
     3901 tacttaacct ttttattcat tggggtgaat tgcaagttca ccatagtttt tccacacaac
     3961 caaaaaggaa actggaaaaa tgttccttct aattaccatt attgcccgtc aagctcagat
     4021 ttaaattggc ataatgactt aataggcaca gccttacaag tcaaaatgcc caagagtcac
     4081 aaggctattc aagcagacgg ttggatgtgt catgcttcca aatgggtcac tacttgtgat
     4141 ttccgctggt atggaccgaa gtatataaca cattccatcc gatccttcac tccatctgta
     4201 gaacaatgca aggaaagcat tgaacaaacg aaacaaggaa cttggctgaa tccaggcttc
     4261 cctcctcaaa gttgtggata tgcaactgtg acggatgccg aagcagtgat tgtccaggtg
     4321 actcctcacc atgtgctggt tgatgaatac acaggagaat gggttgattc acagttcatc
     4381 aacggaaaat gcagcaatta catatgcccc actgtccata actctacaac ctggcattct
     4441 gactataagg tcaaagggct atgtgattct aacctcattt ccatggacat caccttcttc
     4501 tcagaggacg gagagctatc atccctggga aaggagggca cagggttcag aagtaactac
     4561 tttgcttatg aaactggagg caaggcctgc aaaatgcaat actgcaagca ttggggagtc
     4621 agactcccat caggtgtctg gttcgagatg gctgataagg atctctttgc tgcagccaga
     4681 ttccctgaat gcccagaagg gtcaagtatc tctgctccat ctcagacctc agtggatgta
     4741 agtctaattc aggacgttga gaggatcttg gattattccc tctgccaaga aacctggagc
     4801 aaaatcagag cgggtcttcc aatctctcca gtggatctca gctatcttgc tcctaaaaac
     4861 ccaggaaccg gtcctgcttt caccataatc aatggtaccc taaaatactt tgagaccaga
     4921 tacatcagag tcgatattgc tgctccaatc ctctcaagaa tggtcggaat gatcagtgga
     4981 actaccacag aaagggaact gtgggatgac tgggcaccat atgaagacgt ggaaattgga
     5041 cccaatggag ttctgaggac cagttcagga tataagtttc ctttatacat gattggacat
     5101 ggtatgttgg actccgatct tcatcttagc tcaaaggctc aggtgttcga acatcctcac
     5161 attcaagacg ctgcttcgca acttcctgat gatgagagtt tattttttgg tgatactggg
     5221 ctatccaaaa atccaatcga gcttgtagaa ggttggttca gtagttggaa aagctctatt
     5281 gcctcttttt tctttatcat agggttaatc attggactat tcttggttct ccgagttggt
     5341 atccatcttt gcattaaatt aaagcacacc aagaaaagac agatttatac agacatagag
     5401 atgaaccgac ttggaaagta actcgcggcc gccctgcaca acagattctt catgtttgaa
     5461 ccaaatcaac ttgtgatatc atgctcaaag aggccttaat tatattttaa tttttaattt
     5521 ttatgaaaaa aactaacagc aatcatggaa gtccacgatt ttgagaccga cgagttcaat
     5581 gatttcaatg aagatgacta tgccacaaga gaattcctga atcccgatga gcgcatgacg
     5641 tacttgaatc atgctgatta caatttgaat tctcctctaa ttagtgatga tattgacaat
     5701 ttgatcagga aattcaattc tcttccgatt ccctcgatgt gggatagtaa gaactgggat
     5761 ggagttcttg agatgttaac atcatgtcaa gccaatccca tctcaacatc tcagatgcat
     5821 aaatggatgg gaagttggtt aatgtctgat aatcatgatg ccagtcaagg gtatagtttt
     5881 ttacatgaag tggacaaaga ggcagaaata acatttgacg tggtggagac cttcatccgc
     5941 ggctggggca acaaaccaat tgaatacatc aaaaaggaaa gatggactga ctcattcaaa
     6001 attctcgctt atttgtgtca aaagtttttg gacttacaca agttgacatt aatcttaaat
     6061 gctgtctctg aggtggaatt gctcaacttg gcgaggactt tcaaaggcaa agtcagaaga
     6121 agttctcatg gaacgaacat atgcaggatt agggttccca gcttgggtcc tacttttatt
     6181 tcagaaggat gggcttactt caagaaactt gatattctaa tggaccgaaa ctttctgtta
     6241 atggtcaaag atgtgattat agggaggatg caaacggtgc tatccatggt atgtagaata
     6301 gacaacctgt tctcagagca agacatcttc tcccttctaa atatctacag aattggagat
     6361 aaaattgtgg agaggcaggg aaatttttct tatgacttga ttaaaatggt ggaaccgata
     6421 tgcaacttga agctgatgaa attagcaaga gaatcaaggc ctttagtccc acaattccct
     6481 cattttgaaa atcatatcaa gacttctgtt gatgaagggg caaaaattga ccgaggtata
     6541 agattcctcc atgatcagat aatgagtgtg aaaacagtgg atctcacact ggtgatttat
     6601 ggatcgttca gacattgggg tcatcctttt atagattatt acactggact agaaaaatta
     6661 cattcccaag taaccatgaa gaaagatatt gatgtgtcat atgcaaaagc acttgcaagt
     6721 gatttagctc ggattgttct atttcaacag ttcaatgatc ataaaaagtg gttcgtgaat
     6781 ggagacttgc tccctcatga tcatcccttt aaaagtcatg ttaaagaaaa tacatggccc
     6841 acagctgctc aagttcaaga ttttggagat aaatggcatg aacttccgct gattaaatgt
     6901 tttgaaatac ccgacttact agacccatcg ataatatact ctgacaaaag tcattcaatg
     6961 aataggtcag aggtgttgaa acatgtccga atgaatccga acactcctat ccctagtaaa
     7021 aaggtgttgc agactatgtt ggacacaaag gctaccaatt ggaaagaatt tcttaaagag
     7081 attgatgaga agggcttaga tgatgatgat ctaattattg gtcttaaagg aaaggagagg
     7141 gaactgaagt tggcaggtag atttttctcc ctaatgtctt ggaaattgcg agaatacttt
     7201 gtaattaccg aatatttgat aaagactcat ttcgtcccta tgtttaaagg cctgacaatg
     7261 gcggacgatc taactgcagt cattaaaaag atgttagatt cctcatccgg ccaaggattg
     7321 aagtcatatg aggcaatttg catagccaat cacattgatt acgaaaaatg gaataaccac
     7381 caaaggaagt tatcaaacgg cccagtgttc cgagttatgg gccagttctt aggttatcca
     7441 tccttaatcg agagaactca tgaatttttt gagaaaagtc ttatatacta caatggaaga
     7501 ccagacttga tgcgtgttca caacaacaca ctgatcaatt caacctccca acgagtttgt
     7561 tggcaaggac aagagggtgg actggaaggt ctacggcaaa aaggatggag tatcctcaat
     7621 ctactggtta ttcaaagaga ggctaaaatc agaaacactg ctgtcaaagt cttggcacaa
     7681 ggtgataatc aagttatttg cacacagtat aaaacgaaga aatcgagaaa cgttgtagaa
     7741 ttacagggtg ctctcaatca aatggtttct aataatgaga aaattatgac tgcaatcaaa
     7801 atagggacag ggaagttagg acttttgata aatgacgatg agactatgca atctgcagat
     7861 tacttgaatt atggaaaaat accgattttc cgtggagtga ttagagggtt agagaccaag
     7921 agatggtcac gagtgacttg tgtcaccaat gaccaaatac ccacttgtgc taatataatg
     7981 agctcagttt ccacaaatgc tctcaccgta gctcattttg ctgagaaccc aatcaatgcc
     8041 atgatacagt acaattattt tgggacattt gctagactct tgttgatgat gcatgatcct
     8101 gctcttcgtc aatcattgta tgaagttcaa gataagatac cgggcttgca cagttctact
     8161 ttcaaatacg ccatgttgta tttggaccct tccattggag gagtgtcggg catgtctttg
     8221 tccaggtttt tgattagagc cttcccagat cccgtaacag aaagtctctc attctggaga
     8281 ttcatccatg tacatgctcg aagtgagcat ctgaaggaga tgagtgcagt atttggaaac
     8341 cccgagatag ccaagtttcg aataactcac atagacaagc tagtagaaga tccaacctct
     8401 ctgaacatcg ctatgggaat gagtccagcg aacttgttaa agactgaggt taaaaaatgc
     8461 ttaatcgaat caagacaaac catcaggaac caggtgatta aggatgcaac catatatttg
     8521 tatcatgaag aggatcggct cagaagtttc ttatggtcaa taaatcctct gttccctaga
     8581 tttttaagtg aattcaaatc aggcactttt ttgggagtcg cagacgggct catcagtcta
     8641 tttcaaaatt ctcgtactat tcggaactcc tttaagaaaa agtatcatag ggaattggat
     8701 gatttgattg tgaggagtga ggtatcctct ttgacacatt tagggaaact tcatttgaga
     8761 aggggatcat gtaaaatgtg gacatgttca gctactcatg ctgacacatt aagatacaaa
     8821 tcctggggcc gtacagttat tgggacaact gtaccccatc cattagaaat gttgggtcca
     8881 caacatcgaa aagagactcc ttgtgcacca tgtaacacat cagggttcaa ttatgtttct
     8941 gtgcattgtc cagacgggat ccatgacgtc tttagttcac ggggaccatt gcctgcttat
     9001 ctagggtcta aaacatctga atctacatct attttgcagc cttgggaaag ggaaagcaaa
     9061 gtcccactga ttaaaagagc tacacgtctt agagatgcta tctcttggtt tgttgaaccc
     9121 gactctaaac tagcaatgac tatactttct aacatccact ctttaacagg cgaagaatgg
     9181 accaaaaggc agcatgggtt caaaagaaca gggtctgccc ttcataggtt ttcgacatct
     9241 cggatgagcc atggtgggtt cgcatctcag agcactgcag cattgaccag gttgatggca
     9301 actacagaca ccatgaggga tctgggagat cagaatttcg actttttatt ccaagcaacg
     9361 ttgctctatg ctcaaattac caccactgtt gcaagagacg gatggatcac cagttgtaca
     9421 gatcattatc atattgcctg taagtcctgt ttgagaccca tagaagagat caccctggac
     9481 tcaagtatgg actacacgcc cccagatgta tcccatgtgc tgaagacatg gaggaatggg
     9541 gaaggttcgt ggggacaaga gataaaacag atctatcctt tagaagggaa ttggaagaat
     9601 ttagcacctg ctgagcaatc ctatcaagtc ggcagatgta taggttttct atatggagac
     9661 ttggcgtata gaaaatctac tcatgccgag gacagttctc tatttcctct atctatacaa
     9721 ggtcgtatta gaggtcgagg tttcttaaaa gggttgctag acggattaat gagagcaagt
     9781 tgctgccaag taatacaccg gagaagtctg gctcatttga agaggccggc caacgcagtg
     9841 tacggaggtt tgatttactt gattgataaa ttgagtgtat cacctccatt cctttctctt
     9901 actagatcag gacctattag agacgaatta gaaacgattc cccacaagat cccaacctcc
     9961 tatccgacaa gcaaccgtga tatgggggtg attgtcagaa attacttcaa ataccaatgc
    10021 cgtctaattg aaaagggaaa atacagatca cattattcac aattatggtt attctcagat
    10081 gtcttatcca tagacttcat tggaccattc tctatttcca ccaccctctt gcaaatccta
    10141 tacaagccat ttttatctgg gaaagataag aatgagttga gagagctggc aaatctttct
    10201 tcattgctaa gatcaggaga ggggtgggaa gacatacatg tgaaattctt caccaaggac
    10261 atattattgt gtccagagga aatcagacat gcttgcaagt tcgggattgc taaggataat
    10321 aataaagaca tgagctatcc cccttgggga agggaatcca gagggacaat tacaacaatc
    10381 cctgtttatt atacgaccac cccttaccca aagatgctag agatgcctcc aagaatccaa
    10441 aatcccctgc tgtccggaat caggttgggc caattaccaa ctggcgctca ttataaaatt
    10501 cggagtatat tacatggaat gggaatccat tacagggact tcttgagttg tggagacggc
    10561 tccggaggga tgactgctgc attactacga gaaaatgtgc atagcagagg aatattcaat
    10621 agtctgttag aattatcagg gtcagtcatg cgaggcgcct ctcctgagcc ccccagtgcc
    10681 ctagaaactt taggaggaga taaatcgaga tgtgtaaatg gtgaaacatg ttgggaatat
    10741 ccatctgact tatgtgaccc aaggacttgg gactatttcc tccgactcaa agcaggcttg
    10801 gggcttcaaa ttgatttaat tgtaatggat atggaagttc gggattcttc tactagcctg
    10861 aaaattgaga cgaatgttag aaattatgtg caccggattt tggatgagca aggagtttta
    10921 atctacaaga cttatggaac atatatttgt gagagcgaaa agaatgcagt aacaatcctt
    10981 ggtcccatgt tcaagacggt cgacttagtt caaacagaat ttagtagttc tcaaacgtct
    11041 gaagtatata tggtatgtaa aggtttgaag aaattaatcg atgaacccaa tcccgattgg
    11101 tcttccatca atgaatcctg gaaaaacctg tacgcattcc agtcatcaga acaggaattt
    11161 gccagagcaa agaaggttag tacatacttt accttgacag gtattccctc ccaattcatt
    11221 cctgatcctt ttgtaaacat tgagactatg ctacaaatat tcggagtacc cacgggtgtg
    11281 tctcatgcgg ctgccttaaa atcatctgat agacctgcag atttattgac cattagcctt
    11341 ttttatatgg cgattatatc gtattataac atcaatcata tcagagtagg accgatacct
    11401 ccgaaccccc catcagatgg aattgcacaa aatgtgggga tcgctataac tggtataagc
    11461 ttttggctga gtttgatgga gaaagacatt ccactatatc aacagtgttt ggcagttatc
    11521 cagcaatcat ttccgattag gtgggaggct atttcagtaa aaggaggata caagcagaag
    11581 tggagtacta gaggtgatgg gctcccaaaa gatacccgaa tttcagactc cttggcccca
    11641 atcgggaact ggatcagatc tttggaattg gtccgaaacc aagttcgtct aaatccattc
    11701 aataagatct tgttcaatca gctatgtcgt acagtggata atcatttgaa gtggtcaaat
    11761 ttgcgaaaaa acacaggaat gattgaatgg atcaatgggc gaatttcaaa agaagaccgg
    11821 tctatactga tgttgaagag tgacctacat gaggaaaact cttggagaga ttaaaaaatc
    11881 aggaggagac tccaaacttt aagtatgaaa aaaactttga tccttaagac cctcttgtgg
    11941 tttttatttt tttatctggt tttgtggtct tcgtgggtcg gcatggcatc tccacctcct
    12001 cgcggtccga cctgggcatc cgaaggagga cgtcgtccac tcggatggct aagggagagc
    12061 tcggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag
    12121 caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa
    12181 ggaggaacta tatccggatc gagatcctct agagtcgacc tgcaggcatg caagcttgta
    12241 ttctatagtg tcacctaaat cgtatgtgta tgatacataa ggttatgtat taattgtagc
    12301 cgcgttctaa cgacaatatg tacaagccta attgtgtagc atctggctta ctgaagcaga
    12361 ccctatcatc tctctcgtaa actgccgtca gagtcggttt ggttggacga accttctgag
    12421 tttctggtaa cgccgtcccg cacccggaaa tggtcagcga accaatcagc agggtcatcg
    12481 ctagccagat cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg
    12541 ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc
    12601 tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg
    12661 gcgccatctc cttgcaccat tccttgcggc ggcggtgctc aacggcctca acctactact
    12721 gggctgcttc ctaatgcagg agtcgcataa gggagagcgt cgaatggtgc actctcagta
    12781 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
    12841 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
    12901 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
    12961 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
    13021 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
    13081 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
    13141 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
    13201 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
    13261 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
    13321 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
    13381 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
    13441 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
    13501 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
    13561 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
    13621 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
    13681 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
    13741 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
    13801 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
    13861 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
    13921 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
    13981 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
    14041 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
    14101 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
    14161 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
    14221 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
    14281 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
    14341 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
    14401 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
    14461 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
    14521 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
    14581 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
    14641 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
    14701 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    14761 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
    14821 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
    14881 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
    14941 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
    15001 gcagggggat ctcgatcccg cgaaattaat acgactcact atagg
//